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The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. EUROPE. S. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The SpectraMax ABS Plus reader utilizes Molecular . SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Table of Contents. The following document is intended to demonstrate setup of this. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. 0 (IBM Corporation, Armonk, NY, USA). Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. Description 1. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. 3: The cuvette chamber. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. There were 12 replicate wells per dilution in both 96-well and 384- well plates. 72 MB. Our absorbance plate readers feature our PathCheck Sensor. UV/Vis absorbance and fluorescence spectra were measured on SpectraMax® M5 Multi- Mode Microplate Reader. Lift up the lid over the chamber to insert or remove a cuvette. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. 6. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Spectranet customer portal. SoftMax Pro 5 GxP Software provides all the data collection and analysis tools of the standard edition, along withMolecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. Purchase Overlord with the required number of. Example: [Drawer]. Open the Softmax Pro software and select the FP option. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Dual monochromators provide the ability for. 3: The cuvette chamber. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a. Table of Contents. The M5 can read volumes down to 2uL with the uMax Low Volume. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. 313 reference gain check fail. 1. 190-1000 nm, tunable in 1 nm increments. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Example: [Drawer]. 5. The user manuals cover installation, troubleshooting, and validation of the SpectraMax M5 and its accessories. 3. The assay applies fluorescence polarization to quantify Fc-containing IgG. Agilent VWorks Automation Control soft-ware manages all processes and incubation2 Luminescence integration time For supported SpectraMax M5 microplate readers (minimum firmware requirements apply), integration times of 3, 5, 7, and 10 seconds were added for luminescence read mode. HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. 多功能酶标仪SpectraMax M5e. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. ) Click the "OK" button on SoftMax Pro Setup dialog box . SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 3. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. The Spectr. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. 3. PAINS analysisThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. edu) to be added as a trained user in iLab. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images were obtained with SU8010 field. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. D 47 7. . Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. . . Examples: “Press carefully to engage,” “ Do not. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. When the correct COM port is selected, the. 2. SOFTmax PRO UserÕs Manual iii Contents Figures . View the Molecular Devices websiteSpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Table of Contents. . Description. The day after, a final concentration of 1 μmol/L ARS1620, 20 nmol/L SMARTpool siRNA or the combination of the 2 was added in 5 replicates (wells). e. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. The following document is. Softmax Pro GxP Software 6. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The assay window spanned four orders of magnitude. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. . . Page: 1/6. The ratioof the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation of Delta F (%)As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Last lab had ours for years and never had a problem with it. M5. Sale. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. When a feature or capability applies to only certain readers, this exception is noted. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance (ANOVA) (Table 1). Table of Contents. Detection of pro-inflammatory factors. . Five preferred detection modes. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. Select a rugged, lightweight model designed to be used in the field. free tracer decreases, resulting in an. TR-FRET assay. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 72 MB. deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. SoftMax Pro. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. The SoftMax® Touch Software is installed in. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 00 USD. 190-1000 nm, tunable in 1 nm increments. 313 reference gain check fail. Wide range of applications. Description 1. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax M2 and M2e are versatile and reliable. SpectraMax M3, M4, and M5/M5e readers. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidePlace the plate into a SpectraMax M5 e plate reader (Molecular Devices). 4. 3. Figure 4: Transcreener ADP. The excitation and the emission wavelengths on. Control Panel The control panel houses the instrument status LEDs and the microplate. The following document is intended to demonstrate setup of this instrument. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. LUMINESCENCE The SpectraMax M2 and SpectraMax M2e are microplate spectrofluorometers with photomultiplier tube detection. When the user applies a set temperature of 37°C, the top is heated to 37. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The M5 can read volumes down to 2uL with the uMax Low. Step 2. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 9000-0756. 筛选时,SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机,允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长,而无需额外再选购滤光片配件。这篇应 The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Figure 2. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Cavicide (Warehouse, Cat # 79300360) 6. 1. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the emission at 460 nm over 1 h. Consistent results are obtained over time offering workflow flexibility. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Sale. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. . Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. 0 mL). Cell viability was assessed by CellTiterGlo (Promega) reagent 96 hours after beginning treatment, and luminescence was measured on a Spectramax M5 spectrophotometer. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. . The M5 can read volumes down to 2uL with the uMax Low Volume Plate. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. edu) to be added as a trained user in iLab. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader. The SpectraMax M5 is a microplate reader that can be used for various applications, such as ELISA, Western blot, or cell culture. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. . 1. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. SpectraMax M5e Multi-Mode Microplate Reader. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. Sensitive fluorescent quantitation of DNA with the Quant-iT. Statistical analyses The statistical analyses were performed using Graph Prism version 7. 4. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. The absorbance of MPO was calculated using the SpectraMax M5 (Molecular Devices Corporation, San Jose, CA, USA) at 460 nm. 7. 72 MB. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. The following document is intended to demonstrate setup of this. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Setup recommendations for SpectraMax® M5e. The dynamic range of the assay was approximately 5 orders of magnitude. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic Gq-coupled receptor. 6 lbs. Figure 1. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. 40266 Email: drugdiscoverytech@lifetech. com A. 1) EarlyTox NucView 488 Assay (6. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Note: In this user guide, all references to SpectraMax Multi-Mode. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. . Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. . HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax M5, Analyst HT or Analyst GT. STAKMAX. Description 1. . The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. , ~488 nm), has the optical. Customer. For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. The drawing shows a standard microplate of 15 mm height covered with a lid. The IP-One assay can be used to characterize compounds that act on Gq-coupled receptors in either adherent or suspension cells. SpectraMax Spectrofluorometers. edu) to be added as a trained user in iLab. spr SpectraMax iD3 SpectraTest ABS2 v1. VWR offers biophotometers for your lab's needs. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 5. The guide also covers the software features, the specifications, and the accessories of the systems. ISM Equipment Classification (Group 1, Class A) This equipment is. We would like to show you a description here but the site won’t allow us. used. 4. Choose from a three- (M3), four- (M4), or SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity,. 304 signal level saturation. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Our absorbance plate readers feature our PathCheck Sensor technology and. Fluorescence Microplate Reader Comparison Chart. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax Microplate Reader platform. Fluorescent Polarization: Solid black plates, as above. The excitation and the emission. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. 1). Spectramax M5 plate reader (96/384 format) Multimode photometrics for endpoint or kinetic measurements. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. HEALTH AND SAFETY CONSIDERAIONSThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). It is available to all researchers. . SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 0 out of 5 $ 5,999. Open the SoftwarePro® Data Acquisition & Analysis software,. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Examples: “Press carefully to engage,” “ Do not press down. For example, microplates containing different samples can be read using the same orOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Here is a Molecular Devices Spectramax M5 Microplate Reader for sale. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Maximizes fluorescent signal from a wide range of cell types by reducing cell wash artifacts. Step 1. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. . Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M5 Multi-Mode Microplate Reader. With optimized reagents, option to operate the readers. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. 発光モードに対応した機種(SpectraMax M5e/M5/M4/M3)では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. Plate chamber design of the SpectraMax category 1 microplate readers. . Refer to “Installing Detection Cartridges” in. In this. The analysis capabilities ofThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. Wavelength Range. These robust readers have been placed in labs from Antarctica to the. 2. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. acquired on a SpectraMax M5 (Molecular Devices). The SpectraMax M5 Multi-Mode. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Published Dec 16, 2020 | Updated Dec 16, 2020. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 7°C (gradient heating design). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. A wireless light sensor (701-999, PS-3212) was purchased from PASCO. D 1. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. spr SpectraMax Plus 384. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. , ~488 nm), has the optical. The SpectraMax M5 Microplate Reader uses SoftMax® Pro. 0 mm wide, and heights from 14 mm to 15 mm. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. New drivers take 6 days to write, existing drivers take 2 days to convert to Windows 7/10 compatibility. SoftMax Pro software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. ® reader to the computer. Step 2. Molecular Device SpectraMax M5 Plate Reader - Certified with Warranty. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Fix: While proper use of the Shake button is to hold the button down for the duration of the desired mixing time, repeated sequential clicking is now allowed. 315 can't find zero order. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. 1. . Catalog Number: M5. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. EarlyTox Live Cell Assay (6. 315 can't find zero order. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Instructions on the proper use of the SpectraMax M5 Plate Reader. 1. For example, microplates containing different samples can be read using theThe interval between reads was set at 15 s or 60 s, respectively. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. D 1 1. The viscosity solution was prepared by mixing PBS and Glycerol in different proportion. For very concentrated samples, use a minimum2. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. 2. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. 01) between the GFP-transfected and mock-transfected cells at an initial seeding. 306 plate air cal fail,low light. DNA provides the same sensitivity as a spectrophotometer. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. The KeySpan USA-19HS is often required with new Windows 7. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. 1. 7°C (gradient heating design). HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The chamber contains. . 4. There were 12 replicate wells per dilution in both 96-well and 384- well plates.